Contact the University of Washington Clinical Virology Lab (206 685-8037) for ordering instructions. CSF antibody testing will be performed ONLY when paired with a serum sample. Herpes simplex virus (HSV) types 1 and 2 are members of the Herpesviridae family and produce infections that range from mild stomatitis to disseminated and fatal disease. For accurate seroconversion determination, the acute and convalescent samples should be drawn at least 12 -16 weeks apart.įor CSF, see HSV SemiQnt Rapid PCR, Swab/CSF (Herpes Simplex Virus by PCR). However, Ashley-Morrow et al have clearly demonstrated that the HSV-2 IgG Western blot is less sensitive than the HerpeSelect HSV-2 IgG assay for detecting IgG seroconversion following newly acquired HSV-2 infection: the median interval between symptom onset and IgG. It has not been cleared or approved by the US Food and Drug Administration.įor Paired samples, see Herpes Simplex Seroconversion Panel by Western Blot (Paired) for seroconversion on paired serum samples. The HSV-2 IgG Western blot assay has historically been considered the gold standard for identifying HSV-2 IgG reactivity. This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. HSV2 - Positive, Negative or Indeterminate.HSV1 - Positive, Negative or Indeterminate Sputum, biopsy, body fluid, urine, blood, or gastric contents.The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins. Antibodies, which bind to the viral proteins, are detected by an enzyme-mediated color change. The pattern of staining on the two blots (HSV-1 and HSV-2) is dictated by the number and identity of the HSV proteins to which the patient's immune system has antibody. The strips of paper or 'blots' containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. The strips of paper or "blots" containing separated fixed proteins from either HSV-1 or HSV-2 are incubated with the patient's serum. I’m confused on what then supplemental test is saying. I went for a second test w/ reflex testing by labcorp and the index was 1.56 but the REFLEX supplemental test came back NEGATIVE. HSV1 and HSV2 proteins from detergent lysates ("Bernstein's lysate") of HSV infected cells are separated by electrophoresis and transferred to nitrocellulose paper. I was recently tested for HSV 2 on a routine checkup and it came back positive 1.78. The detection of HSV1 and HSV2 IgG class antibodies by Western blot in serum or CSF. This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections with active viral shedding it also differentiates HSV-1.
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